Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 1 The map of the recombinant vector for expression of His-CDH1 fusion peptide. The CDH1 ectodomain was link to the His-tag in the pET-44a(+) vector. Expression of His-CDH1 fusion peptide was driven by T7 lac promoter.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Recombinant, Plasmid Preparation, Expressing

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 2 Analysis of the optimal expressed condition of His-CDH1 fusion peptide by SDS-PAGE. M, protein molecular weight marker; 1, control; 2-9, the sample induced by IPTG at 1, 1.5, 2, 2.5, 3, 4, 5 and 6 h, respectively.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: SDS Page, Molecular Weight, Marker, Control

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 3 Western blot analysis of the condition for purifying His- CDH1 peptide using Ni-NTA chromatography. 1, prestained protein marker; 2, the recovery fluid with lysate supernatant; 3, the recovery fluid with 20 mmol imidazol; 4-5, the recovery fluid with 100 mmol imidazol; 6-7, the recovery fluid with 250 mmol imidazol; 8-9, the recovery fluid with 500 mmol imidazol; 10, the recovery fluid with 1 000 mmol imidazol.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Western Blot, Chromatography, Marker

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 4 Ability of the sheep CDH1 polyclonal antibodies was detected by Western blotting. Total protein was from sheep testis (T), kinedy (K), pulmo (P) and liver (L). There were three bands in 135, 120 and 80 kDa.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Western Blot

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 5 Distribution patterns of CDH1 and PLZF in cross sections of 5-mon-old sheep testis. A, immunohistochemistry examination of PLZF expressing cells within cross sections of seminiferous tubules from 5-mon-old sheep testis. B, immunohistochemistry examination of CDH1 in the same section. C, the nuclei of the cell sections were stained by Hoechst 33342. D, merged image of A, B and C. Some PLZF- and CDH1-positive cells comprising single (diamond arrows) and paired spermatogonia (arrows) were localized at the basement membrane of the seminiferous tubules. Scale bars are 40 μm.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Immunohistochemistry, Expressing, Staining, Membrane

Journal: Journal of Integrative Agriculture

Article Title: CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

doi: 10.1016/s2095-3119(13)60689-9

Figure Lengend Snippet: Fig. 6 Whole-mount immunohistochemistry of 5-mon-old sheep seminiferous tubules. The CDH1-positive cells were small in number, there are single cells attached the basement membrane of the seminiferous tubules (arrows), and there are paired cells attached each other (diamond arrow). Scale bar is 200 μm.

Article Snippet: After blocking with 5% skim milk for 1 h at RT, the seminiferous tubules were incubated with CDH1 polyclonal antibody and anti-PLZF antibody (Santa Cruz) overnight at 4°C.

Techniques: Immunohistochemistry, Membrane

Journal: Oral Science International

Article Title: Down‐regulated expression of family with sequence similarity 3, member B (FAM3B), in oral squamous cell carcinoma

doi: 10.1016/s1348-8643(12)00004-3

Figure Lengend Snippet: Fig. 1. Family with sequence similarity 3, member B (FAM3B) mRNA expression in oral squamous cell carcinoma (OSCC)-derived cell lines. FAM3B mRNA levels were

Article Snippet: Immunohistochemical analysis Immunohistochemical analysis was performed on 4- m sections of paraffin-embedded specimens using anti-FAM3B polyclonal antibody (Proteintech).

Techniques: Sequencing, Expressing, Derivative Assay

Journal: Oral Science International

Article Title: Down‐regulated expression of family with sequence similarity 3, member B (FAM3B), in oral squamous cell carcinoma

doi: 10.1016/s1348-8643(12)00004-3

Figure Lengend Snippet: Fig. 2. Family with sequence similarity 3, member B (FAM3B) protein expression in oral

Article Snippet: Immunohistochemical analysis Immunohistochemical analysis was performed on 4- m sections of paraffin-embedded specimens using anti-FAM3B polyclonal antibody (Proteintech).

Techniques: Sequencing, Expressing

Journal: Oral Science International

Article Title: Down‐regulated expression of family with sequence similarity 3, member B (FAM3B), in oral squamous cell carcinoma

doi: 10.1016/s1348-8643(12)00004-3

Figure Lengend Snippet: Fig. 3. Expression profile of family with sequence similarity 3, member B (FAM3B) mRNA

Article Snippet: Immunohistochemical analysis Immunohistochemical analysis was performed on 4- m sections of paraffin-embedded specimens using anti-FAM3B polyclonal antibody (Proteintech).

Techniques: Expressing, Sequencing

Journal: Oral Science International

Article Title: Down‐regulated expression of family with sequence similarity 3, member B (FAM3B), in oral squamous cell carcinoma

doi: 10.1016/s1348-8643(12)00004-3

Figure Lengend Snippet: Fig. 4. Immunohistochemical (IHC) analysis of family with sequence similarity 3, member B (FAM3B) protein expression in primary oral squamous cell carcinoma (OSCC) samples. Representative results of IHC analysis for FAM3B in normal oral tissue (A, B) and primary OSCC (C, D). Original magnification: ×100 (A, C) and ×400 (B, D). Positive immunoreactions

Article Snippet: Immunohistochemical analysis Immunohistochemical analysis was performed on 4- m sections of paraffin-embedded specimens using anti-FAM3B polyclonal antibody (Proteintech).

Techniques: Immunohistochemical staining, Sequencing, Expressing

Journal: Biomedical research (Tokyo, Japan)

Article Title: Estrogen receptor alpha and beta are both involved in the cerebral VEGF/Akt/NO pathway and cerebral angiogenesis in female mice.

doi: 10.2220/biomedres.31.337

Figure Lengend Snippet: Fig. 2 Analysis of phosphorylated Akt (pAkt) (2A, B), and phosphorylated eNOS (2C) shows decreased expression in frontocortical tissues in WT (Con), ERαKO, and ERβKO mice (A–C). Levels of Akt phosphorylated at Ser473 and Thr308 were determined by ELISA. In immunoblotting, β-actin served as loading control. Values are mean ± S.E.

Article Snippet: The following commercially available antibodies were used for Western blot analysis: a) anti-human VEGF rabbit polyclonal antibody (Immuno-Biological Laboratories, Fujioka, Japan), b) anti-human Flt-1 rabbit polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), c) anti-human KDR rabbit polyclonal antibody (Santa Cruz Biotechnology), d) anti-mouse Ser473-phospho-Akt rabbit polyclonal antibody (Cell Signaling, Beverly, MA, USA), e) anti-human endothelial nitric oxide synthase (eNOS) rabbit polyclonal antibody (Affinity BioReagents, Golden, CO, USA), f) anti-bovine basic fibroblast growth factor (bFGF) mouse monoclonal antibody (Upstate Biotechnology, Lake Placid, NY, USA), g) anti-human Bcl-2 rabbit polyclonal antibody (Santa Cruz Biotechnology), h) anti-Akt rabbit polyclonal antibody (Cell Signaling), i) anti-mouse estrogen receptor alpha rat monoclonal antibody (Novocastra Laboratories, Benton Lane, Newcastle upon Tyne, UK), j) anti-human estrogen receptor beta rabbit polyclonal antibody (Affinity BioReagents), and k) anti-Xenopus laevis beta-actin mouse monoclonal antibody (Abcam, Cambridge, MA, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: International journal of obesity (2005)

Article Title: Obesity impairs skeletal muscle AMPK signaling during exercise: role of AMPKα2 in the regulation of exercise capacity in vivo.

doi: 10.1038/ijo.2010.220

Figure Lengend Snippet: Figure 1 Blood glucose levels during exercise (a), and gastrocnemius glucose uptake (Rg; b), glucose clearance (Kg; c), AMPKa2 activity (d), AMPKa2 Thr172

Article Snippet: Blots were probed with an anti-AMPKa2 goat polyclonal antibody (1:100; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-AMPKa Thr172 rabbit polyclonal antibody (1:100; Cell Signaling, Danvers, MA, USA), or an anti-ACCb Ser221 rabbit polyclonal antibody (1:100; Cell Signaling).

Techniques: Activity Assay